Genetics of Aspergillus flavus: linkage of aflatoxin mutants

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Abstract
Eight aflatoxin (afl) mutants of A. flavus were induced with N-methyl-N''-nitro-N-nitrosoguanidine. Heterozygous diploids formed between afl mutants and tester strains revealed that each afl mutant was recessive. Haploids selected from these heterozygous diploids indicated the linkage of all 8 afl mutants to markers on group VII. These include previously mapped arg-7 (arginine), leu (leucine), dominant afl-1, and nor which accumulates norsolorinic acid that is vislble as an orange-red pigment. Diploid complementation tests indicated that all but 2 afl mutants were nonallelic. Diploids homozygous for nor, resulting from crossing-over, were isolated and used to map new afl genes.