Properties of preparations from the central nervous system which degrade coenzymes I and II; their connexion with carbohydrate metabolism

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Abstract
The system degrading cozymase in prepns. of mammalian brain and spinal cord was extracted by water or by strong salt solns., and separated from some 90% of the nitrogenous constituents of the tissues. Of about 65 materials which were examined for their actions on the breakdown, none caused significant stimulation even with a washed and dialyzed enzyme prepn. Two main groups of compounds inhibited the breakdown when present in concns. comparable to, or less than, those of the cozymase undergoing reaction. These were pyridine and phenazine derivatives. The active pyridine compounds included nicotine and 4(5)-3[image]-pyridyl-glyoxaline as well as nicotinamide. Of the phenazine derivatives, phenosafranine and Janus green were most potent. Correlations existed between the actions of these substances on cozymase degradation and their reported actions on the release of aerobic glycolysis by brain slices.