Novel Enzyme Immunoassays for Specific Detection ofFusarium oxysporumf. sp.cucumerinumand for General Detection of VariousFusariumSpecies

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Abstract
Competitive types of two novel enzyme-linked immunosorbent assays (ELISA) for Fusarium species were developed. Antiserum against a strain (F504) of F. oxysporum was elicited in rabbits, and a highly specific, sensitive, and accurate ELISA for the homologous strain was developed by using the antiserum with .beta.-D-galactosidase-labeled anti-rabbit IgG as the secondary antibody and cell fragments of the strain attached to Amino-Dylark balls as the solid-phase antigen. All other microorganisms tested, including nine other strains of Fusarium, showed little cross-reactivity. When cell fragments of F. oxysporum F501 attached to the balls were used as a solid-phase antigen in a heterologous competitive ELISA, the modified system was a general assay for strains of four Fusarium species with a high sensitivity. The specificity of the heterologous competitive ELISA was shown to be limited to Fusarium species. The reasons for developing the improved ELISA is also presented.

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