RELATIONSHIP BETWEEN RENAL CLEARANCE, PROTEIN-BINDING AND URINE FLOW FOR DIGITOXIN, A COMPOUND OF LOW CLEARANCE IN THE ISOLATED PERFUSED RAT-KIDNEY

  • 1 January 1984
    • journal article
    • research article
    • Vol. 228  (1) , 174-179
Abstract
An isolated perfused rat kidney preparation is described which allows the relationship between protein binding and renal clearance to be studied in a quantitative manner. The influence of unbound fraction of drug in the perfusate, urine pH and urine flow upon the renal clearance of digitoxin is examined. Renal clearance and unbound fraction were related linearly. Urine pH did not influence digitoxin renal clearance but urine flow did, in a nonlinear manner. A simple physiologically based model of renal clearance is developed which predicts the influence of urine flow and protein binding upon digitoxin clearance in this preparation.