Three-dimensional sub–100 nm resolution fluorescence microscopy of thick samples
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- 11 May 2008
- journal article
- Published by Springer Nature in Nature Methods
- Vol. 5 (6) , 527-529
- https://doi.org/10.1038/nmeth.1211
Abstract
Imaging volumes as thick as whole cells at three-dimensional (3D) super-resolution is required to reveal unknown features of cellular organization. We report a light microscope that generates images with translationally invariant 30 x 30 x 75 nm resolution over a depth of several micrometers. This method, named biplane (BP) FPALM, combines a double-plane detection scheme with fluorescence photoactivation localization microscopy (FPALM) enabling 3D sub-diffraction resolution without compromising speed or sensitivity.Keywords
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