Changes in Uptake of S35-Sulfate by Carrageenin Granuloma in Normal and Lathyritic Rats of Different Ages

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Abstract
The effect of aminoacetonitrile (10 mg) 100 g b.w. (day) on the acid mucopolysaccharide metabolism of carrageenin granuloma was studied in rats of different age groups ("young", 1-2 months, "middle-aged", 4-5 months; "adult", 10-12 months). The animals received 25 [mu]C of S35-sulfate 100 g b.w. 48 hr. before being killed at 0, 5, 10, 15 and 20 days. Then after the injection of carregeenin s35-activity was determined in the serum protein, granuloma, liver and skeletal musculature. The changes in hydroxyproline, hexuronic acid, hexosamine contents of the granuloma were also examined. Increased acidmucopoly-saccharide (AMP) incorporation was observed in the young and middle-aged animals on the 5th day of development of the carrageenin granuloma. In the adult animals the same increase could be demonstrated on the 15th day. Aminoacetonitrile inhibited the increase in S35-gulfate uptake and in hexuronic acid content, more in the adult than in the young animals, as compared with their respective controls. In the young group a transitory increase in AMP incorporation was observed on the 5th day. The younger organism is apparently able, at least temporarily, to block the inhibitory effect of lathyrogens on granulation tissue.