An Estimation of β-Glucuronidase and N-Acetyl-β-D-Glucosaminidase Activity in Normal and Chronic Lymphocytic Leukaemia Lymphocytes

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Abstract
The lysosomal enzymes β-glucuronidase (EC 3.2.1.31) and N-acetyl-β-D-glucosaminidase (EC 3.2.1.30) were estimated by biochemical and cytochemical means in the circulating lymphocytes of 20 control subjects, 19 cases of B cell and one case of T cell chronic lymphocytic leukaemia. Significantly lower levels of activity were observed both biochemically and cytochemically for each enzyme in B cell chronic lymphocytic leukaemia lymphocytes, whereas the values obtained for the T cell chronic lymphocytic leukaemic patient fell within the normal range. The absence of staining reaction for both enzymes in the majority of B cell chronic lymphocytic leukaemia lymphocytes contrasted with the uniform pattern of polar positivity obtained in T cell chronic lymphocytic leukaemia lymphocytes.