Two‐dimensional electrophoresis of the plasma proteins of alpacas and llamas: genetic polymorphism of α1B‐glycoprotein and three other proteins
- 1 December 1989
- journal article
- research article
- Published by Wiley in Animal Genetics
- Vol. 20 (s1) , 395-406
- https://doi.org/10.1111/j.1365-2052.1989.tb00895.x
Abstract
Plasma samples of alpacas and llamas were analysed by a simple method of two-dimensional (2-D) agarose gel (pH 8.6)-horizontal polyacrylamide gel (pH 9.0) electrophoresis, followed by general protein staining of gels. Genetic polymorphism in both species is described for .alpha.1B-glycoprotein (.alpha.1B) and three other unidentified proteins designated prealbumin (Pr), postalbumin 1 and 2 (Pa1 and Pa2). .alpha.1B was identified by cross-reactivity with antisera for human and pig .alpha.1B. Altogether, two alleles of Pr, two of Pa1, five of .alpha.1B and three of Pa2 are described. Most of the alleles were present in alpacas and llamas. Alpacas showed a high degree of polymorphism at all four loci. Llamas showed considerable polymorphism at only the Pa1 and Pa2 loci. The theoretical probability of exclusion (PE) of an incorrectly assigned parent was estimated to be aobut 80% in each species by typing for the six polymorhpic plasma proteins reported so far in these species. The given method of 2-D electrophoresis revealed no fixed differences in protein mobilities that discriminate between llamas and alpacas.Keywords
This publication has 13 references indexed in Scilit:
- Simultaneous phenotyping of pig plasma α-protease inhibitors (PI1, PO1A, PO1B, PI2) and four other proteins (PO2, TF, CP, HPX) by a simple method of 2D horizontal electrophoresisAnimal Genetics, 2009
- Polymorphic plasma postalbumins of some domestic animals (pig PO2, horse Xk and dog Pa proteins) identified as homologous to human plasma α1B-glycoproteinAnimal Genetics, 2009
- Three new plasma protein polymorphisms in domestic foxes, detected by a simple method of 2D horizontal electrophoresisHereditas, 2008
- Genetic variation in the blood of llamas, Llama glama, and alpacas, Llama pacosAnimal Genetics, 1988
- Genetic polymorphism of plasma α1B-glycoprotein and transferrin in arctic and silver foxesAnimal Genetics, 1988
- Parentage testing of dogs using variants of blood proteins: description of five new plasma protein polymorphismsAnimal Genetics, 1987
- Genetic polymorphism of human plasma ?1-B-glycoprotein: phenotyping by immunoblotting or by a simple method of 2-D electrophoresisHuman Genetics, 1987
- Karyological conservatism in South American camelidsCellular and Molecular Life Sciences, 1986
- Genetic variation at a pig serum protein locus, Po‐2 and its assignment to the Phi, Hal, S, H, Pgd linkage groupAnimal Blood Groups and Biochemical Genetics, 1983
- The genetics of transferrins in cattleHeredity, 1965