TRIMERIC AND OLIGOMERIC FLAVANOIDS IN BEER: A JOINT APPLICATION OF SEPHADEX LH-20 AND REVERSED-PHASE GRADIENT ELUTION HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

Abstract
Flavanoids were separated from a commercial Belgian beer by adsorption on Sephadex LH-20 and desorption from this gel with an acetone-water mixture (60%). The resulting desorbates were analyzed by reversed-phase gradient elution high-performance liquid chromatography. The results clearly indicate that beer contains trimeric and higher oligomeric proanthocyanidins. These polyphenols are not only important for the colloidal stability of a beer, but also for its flavour.