A METHOD FOR THE ANALYSIS OF URINARY 17-HYDROXYCORTICOSTEROIDS
- 1 March 1961
- journal article
- research article
- Published by Bioscientifica in Journal of Endocrinology
- Vol. 22 (1) , 31-46
- https://doi.org/10.1677/joe.0.0220031
Abstract
SUMMARY: A method for the analysis of urinary 17-hydroxycorticosteroids is described. The urinary steroids are submitted, in situ, to sodium borohydride reduction, sodium periodate oxidation and mild alkaline hydrolysis. The generated 17-ketosteroids are separated by partition chromatography into 11-deoxy and 11-oxy fractions which are separately estimated by the Zimmermann reaction. By this method it is possible to estimate the urinary metabolites of cortisol without interference by corticosteroids not oxygenated at C-11.Keywords
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