The Behavior of Influenza Viruses in Various Tissue Culture Systems
Open Access
- 1 April 1957
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 78 (4) , 233-239
- https://doi.org/10.4049/jimmunol.78.4.233
Abstract
Summary: Comparison of the growth characteristics of first and twelfth monkey kidney (MK) passaged influenza B in the same type of culture indicated that adaptation had taken place after passage. However, the growth of an egg-adapted influenza B, Lee, showed a much lesser degree of adaptation for MK cells than the first passaged virus which had been isolated directly from man. Both MK and egg lines of influenza B agglutinated human type O, fowl, and monkey erythrocytes in a similar manner. With sheep erythrocytes titers of agglutination by the egg-adapted virus were significantly greater than by the MK line. Attempts to propagate influenza A or B in cultures of conjunctival, fibroblast, KB, HeLa or embryonic skin, all of human origin, were uniformly unsuccessful regardless of amount of virus inoculated or previous passage history. Growth of influenza C could not be demonstrated in any of these cells nor in cultures of human embryo lung (HEL), human embryo kidney (HEK), or MK. Evidence of growth of influenza A and B with increments in hemagglutinating and infectious virus as well as cytopathogenic effects in cultures of bovine embryo kidney (BEK) has been presented.Keywords
This publication has 10 references indexed in Scilit:
- Growth Characteristics and Cytopathogenic Effects of Influenza A and B in Cultures of Human Embryo TissuesThe Journal of Immunology, 1956
- Isolation and Cytopathogenic Effect of Influenza B Viruses in Monkey Kidney Cultures.Experimental Biology and Medicine, 1955
- Propagation in a Fluid Medium of a Human Epidermoid Carcinoma, Strain KBExperimental Biology and Medicine, 1955
- THE SPECIFIC AMINO ACID REQUIREMENTS OF A HUMAN CARCINOMA CELL (STRAIN HELA) IN TISSUE CULTUREThe Journal of Experimental Medicine, 1955
- A NON-TRANSMISSIBLE CYTOPATHOGENIC EFFECT OF INFLUENZA VIRUS IN TISSUE CULTURE ACCOMPANIED BY FORMATION OF NON-INFECTIOUS HEMAGGLUTININSThe Journal of Experimental Medicine, 1955
- Continuous Subcultivation of Epithelial-like Cells from Normal Human Tissues.Experimental Biology and Medicine, 1954
- Monolayer Tissue Cultures I. Preparation and Standardization of Suspensions of Trypsin-Dispersed Monkey Kidney Cells.Experimental Biology and Medicine, 1954
- INFLUENZA VIRUS-ERYTHROCYTE INTERACTION .2. EFFECT OF EGG PASSAGE ON THE REACTION BETWEEN A RECENTLY RECOVERED INFLUENZA-A VIRUS AND SHEEP ERYTHROCYTES1954
- Method for Establishing Human Cells in Culture: Susceptibility to Poliomyelitis after Prolonged Cultivation.Experimental Biology and Medicine, 1953
- Nutrition of Animal Cells in Tissue Culture. I. Initial Studies on a Synthetic Medium.,Experimental Biology and Medicine, 1950