Optimization of a protective medium for enhancing the viability of freeze-dried Lactobacillus delbrueckii subsp. bulgaricus based on response surface methodology
- 22 October 2005
- journal article
- Published by Oxford University Press (OUP) in Journal of Industrial Microbiology & Biotechnology
- Vol. 33 (1) , 55-61
- https://doi.org/10.1007/s10295-005-0041-8
Abstract
Response surface methodology (RSM) was used to optimize a protective medium for enhancing the cell viability of Lactobacillus delbrueckii subsp. bulgaricus LB14 during freeze-drying. Using a previous Plackett–Burman design, it was found that sucrose, glycerol, sorbitol and skim milk were the most effective freeze-drying protective agents for L. bulgaricus LB14. A full factorial central composite design was applied to determine the optimum levels of these four protective agents. The experimental data allowed the development of an empirical model (PL. bulgaricus LB14 freeze-dried in this medium obtained a cell viability of up to 86.53%.Keywords
This publication has 23 references indexed in Scilit:
- Statistical optimization of media for extracellular polysaccharide by Pholiota squarrosa (Pers. ex Fr.) Quel. AS 5.245 under submerged cultivationBiochemical Engineering Journal, 2004
- Optimization of d-lactic acid production by Lactobacillus coryniformis using response surface methodologyFood Microbiology, 2004
- Relevant factors for the preparation of freeze-dried lactic acid bacteriaInternational Dairy Journal, 2004
- Influence of trehalose and moisture content on survival of Lactobacillus salivarius subjected to freeze-drying and storageProcess Biochemistry, 2003
- Response surface methodological approach to optimize the nutritional parameters for neomycin production by Streptomyces marinensis under solid-state fermentationProcess Biochemistry, 2003
- The Trehalose Myth Revisited: Introduction to a Symposium on Stabilization of Cells in the Dry StateCryobiology, 2001
- Effect of protective solutes on leakage from and survival of immobilized Lactobacillus subjected to drying, storage and rehydrationJournal of Applied Microbiology, 1999
- Carbohydrates and the dehydration inactivation of Lactobacillus plantarum: The role of moisture distribution and water activityJournal of Food Engineering, 1997
- Physiological response of Enterococcus faecalis JH2-2 to cold shock: growth at low temperatures and freezing/thawing challengeLetters in Applied Microbiology, 1996
- Thermoprotective effect of sorbitol on proteins during dehydrationJournal of Agricultural and Food Chemistry, 1993