Influence of IBA and Cordycepin on Rooting and RNA Synthesis in Stem Cuttings of Phaseolus aureus Roxb

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Abstract
Adventitious roots are initiated on stem cuttings of Phaseolus aureus Roxb. by treatment with IBA for 24 h, although subsequent transfer to boric acid is essential for their development. Cordycepin enhances auxin-induced rooting when supplied for 4 h within the first twenty hours of IBA treatment, but not thereafter. Cordycepin alone does not enhance rooting. IBA treatment of cuttings for 12 h results in a marked inhibition of RNA synthesis, including poly(A)-rich RNA, in the hypocotyl. After 24 h treatment RNA synthesis is seen to increase, with a more marked recovery in the synthesis of poly(A)+RNA relative to other RNAs. Subsequent transfer to boric acid maintains this recovery. Cordycepin does not inhibit RNA synthesis below the level induced by IBA although on subsequent transfer to boric acid is seen to enhance synthesis and turnover of both polyadenylated and non-polyadenylated RNA.