A Microprocedure for the Determination of 4-Pyridoxic Acid in Urine
- 1 June 1964
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 10 (6) , 479-489
- https://doi.org/10.1093/clinchem/10.6.479
Abstract
A microprocedure for the estimation of urinary 4-pyridoxic acid which is apparently specific, yet simple enough for routine clinical use, has been adapted from the Fujita et al. (16-17) and Reddy et al. (22) procedures. Average values for urinary 4-pyridoxic acid for 7 women ranged from 3.8 to 6.9 µM/24 hr. by this method. These values are compatible with average daily intakes of vitamin B6 and indicate that by this method interfering fluorescent substances have been removed by chromatography. Good agreement was obtained with replicate analyses.Keywords
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