THE IDENTIFICATION OF PITUITARY INTERSTITIAL CELL STIMULATING HORMONE IN HUMAN URINE

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Abstract
THE purpose of this communication is to describe a method for the preparation and identification of pituitary interstitial cell stimulating (or luteinizing) hormone in human urine. Presumptive evidence for the presence of interstitial cell stimulating hormone (ICSH) in urine extracts has existed for many years. The presence of ICSH is implied in all extracts which give a positive test for gonadotropins by conventional bio-assay procedures depending upon uterine or ovarian enlargement, since ICSH is necessary for the completion of follicular growth and for estrogen secretion. However, a female test animal cannot at present be employed for a quantitative assay of either follicle stimulating hormone (FSH) or ICSH (1) in an unfractionated gonadotropin preparation, such as a urine concentrate, because in this sex the two hormones appear to be inextricably related in the expression of their physiological effects. It was not until the demonstration that in the male animal synergism between FSH and ICSH does not pertain to all structures responding to these hormones that the development of an actual assay for ICSH became theoretically possible