STUDIES ON PHOSPHOLIPIDS. 9. THE COMPOSITION OF RAT-LIVER LECITHINS

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Abstract
Lecithin was isolated from dinitrophenylated rat-liver lipids by chromatography on diethylaminoethylcellulose, with cold acetone as solvent. The lecithin fraction was purified by counter -current distribution in carbon tetrachloride-methanol-water and analysed for phosphorus and fatty acid. A mathematical analysis enabled an estimate of the probable complete composition to be made. Stearoyl arachidonoyl lecithin was the major component. After exchange of inorganic [p32]phosphate for 1 hr., the stearoyl lecithins were calculated to have an average specific radioactivity of 81 [plus or minus] 32 counts/min./[mu]g atom of phosphorus as compared with 756 [plus or minus] 24 counts/min./[mu]g atom of phosphorus for the palmitoyl lecithins.