Effect of an 8-hour holding period on in vivo and in vitro properties of red cells and factor VIII content of plasma after collection in a red cell additive system

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Abstract
Extension of the holding time for whole blood units from 6 to 8 hours at ambient temperature should provide enhanced flexibility in the preparation of platelet concentrates (PCs). A paired study was conducted to evaluate the characteristics of stored red cells (RBCs) and plasma prepared from whole blood collected into a red cell additive system (CPD‐ADSOL) after an extended holding time. An individual donated a unit of whole blood on two occasions; 1 unit was held for 6 hours before processing and the other for 8 hours. Autologous RBC 24‐ hour survival levels after 42 days of storage were comparable. Laboratory A, using a 99mTc‐51Cr technique, found mean survival levels of 79 percent (6‐hour hold) and 78 percent (8‐hour hold) (n = 8). Analysis by the single‐label procedure found the mean levels to be 82 and 81 percent. Laboratory B, using an albumin 125I‐51Cr technique, found mean survival levels of 74 and 72 percent (n = 10). Mean hemolysis and ATP levels were found to be comparable after 42 days of storage following 6‐ and 8‐hour holding periods. 2,3 DPG levels were reduced to a greater degree during the longer hold. The factor VIII levels in plasma frozen for at least a month after 6‐ and 8‐hour holding periods were comparable; thawed plasma contained mean levels of 0.77 and 0.76 units per mL (n = 21). These studies indicate that components prepared by using a CPD‐ADSOL system after holding periods of 6 and 8 hours have comparable properties.